Our lab tries to adhere to the principle of Open Chemistry (Lavis, 2021). We aspire to share reagents and synthesize custom probes for academic scientists at cost price.
Currently, we are offering a complimentary batch of FluoroProx probes to the scientific community. First come, first served.
To request these items, simply add them to your "request cart" and proceed to checkout. Upon checkout, your selected items and shipping details will be automatically copied to your clipboard. Next, paste this information into an email and send it to us at 📨furthscilifelab.uu.se.
Please ensure to include "Sample Request" in the subject line.
By default, we ship items via regular mail, but if you have a preferred carrier, please include that information in your email.
Probes
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FluoroProx488
Amount: 10 μL @1 mM in DMSO
Reactions: 50-200 (50-200 μL Vol)
Mol. weight: 1685.83 g/mol
Ex/Em: 496 nm / 515 nm
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FluoroProx594
Amount: 10 μL @1 mM in DMSO
Reactions: 50-200 (50-200 μL Vol)
Mol. weight: 1869.10 g/mol
Ex/Em: 593 nm / 613 nm
Molecular ruler 1.4 nm
Amount: 10 μL @1 mM in DMSO
Reactions: 50-200 (50-200 μL Vol)
Mol. weight: 675.8 g/mol
End-to-end length: ~1.4 nm
Plasmids
This section serves as a reference for lab members to access information on plasmid availability and microscope images. It aids in anticipating experimental outcomes such as expression and localization. For plasmid distribution, please refer to Addgene.
| Plasmid | ID | Description | Image |
|---|---|---|---|
| CAG-NLS-GFP | 104061 | GFP localized to nucleus trough N-term NLS from SV40. Bacterial Resistance: Ampicillin, 100 μg/mL Growth Temperature: 30°C Strain: NEB Stable |
 
24h (left) and 72h (right). Notice the shift in signal to nucleoli. PC3 cells. |
| pCMV-GFP (pmaxGFP) | pmaxGFP | GFP, CMV promotor, a SV40 pA, a pUC ori and the kanamycin gene. |
|
| pCAG-Palm-GFP | 14757 | GFP localized to membrane through N-term palmitoylation signal from GAP43. Bacterial Resistance: Ampicillin, 100 μg/mL Growth Temperature: 37°C Strain: DH5alpha |
|
| CMV -Ecad-EGFP |
28009 | EGFP at C-terminal of Human Ecadherin in pcDNA3.1. The first two amino acids of the standard EGFP sequence (Met-Val) are missing due to the cloning strategy. There is also a Y238I mutation present at the C-terminus of EGFP. These differences do not affect the fluorescent protein properties. Bacterial Resistance: Ampicillin, 100 μg/mL Growth Temperature: 37°C Strain: DH5alpha 👩🏼💻 Examine plasmid directly in web-browser (SeqViz) 🧬 Download SnapGene file addgene-plasmid-28009-sequence-181212.dna |
|
| pCMV-FLAG-Omomyc | 113168 |
Omomyc tagged with a FLAG-tag at the N terminal. Shown here stained with Rabbit anti-Flag as primary and Goat anti-Rabbit Alexa647 as secondary. Bacterial Resistance: Ampicillin, 100 μg/mL Growth Temperature: 37°C Strain: DH5alpha 👩🏼💻 Examine plasmid directly in web-browser (SeqViz) 🧬 Download SnapGene file addgene-plasmid-113168-sequence-217924.dna |
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