Our lab tries to adhere to the principle of Open Chemistry (Lavis, 2021). We aspire to share reagents and synthesize custom probes for academic scientists at cost price.

Currently, we are offering a complimentary batch of FluoroProx probes to the scientific community. First come, first served.
To request these items, simply add them to your "request cart" and proceed to checkout. Upon checkout, your selected items and shipping details will be automatically copied to your clipboard. Next, paste this information into an email and send it to us at 📨furthscilifelab.uu.se.
Please ensure to include "Sample Request" in the subject line.

By default, we ship items via regular mail, but if you have a preferred carrier, please include that information in your email.

Probes

Plasmids

This section serves as a reference for lab members to access information on plasmid availability and microscope images. It aids in anticipating experimental outcomes such as expression and localization. For plasmid distribution, please refer to Addgene.

Universal sequencing primers

Universal primers play a crucial role in molecular biology techniques, particularly in Sanger sequencing and vector construction, by providing a reliable means of amplifying and analyzing a variety of sequences. 

Primer Name	Sequence (5' -> 3')	Length [bases]	Tm [°C]	GC [%]
-96gIII	CCC TCA TAG TTA GCG TAA CG	20	57.3	50
1392r	ACG GGC GGT GTG TGT AC	17	57.6	65
1492r	TAC GGT TAC CTT GTT ACG ACT T	22	56.5	41
27f	AGA GTT TGA TCA TGG CTC A	19	52.4	42
5AOX	GAC TGG TTC CAA TTG ACA AGC	21	57.9	48
ACYCDuetUP1	GGA TCT CGA CGC TCT CCC T	19	61.0	63
BAC1	GCT CGT ATG TTG TGT GGA ATT G	22	58.4	45.5
CMVfor	CGC AAA TGG GCG GTA GGC GTG	21	65.7	67
CMVmin	CGC CAT CCA CGC TGT TTT G	19	58.8	58
DuetDOWN1	GAT TAT GCG GCC GTG TAC AA	20	57.3	50
DuetUP2	TTG TAC ACG GCC GCA TAA TC	20	57.3	50
EGFP-Cfor	GAT CAC ATG GTC CTG CTG	18	56	55.6
EGFP-Nrev	CCG TCC AGC TCG ACC AG	17	60	70.6
EuVH	GGC AGC AGC CAC AGG TAA GA	20	61.4	60
EuVL	TTG CTG TTG CAC AGT GAT TC	20	55.2	45
Gal4AD	TAC CAC TAC AAT GGA TG	17	47.9	41
Gal4BD	TCA TCG GAA GAG AGT AG	17	50.4	47
HuCAL_VH_for	GAT AAG CAT GCG TAG GAG AAA	21	55.9	43
IgG_const_for	AGC CCA GCA ACA CCA AGG	18	58.2	61
IntXL39	ATT AGG ACA AGG CTG GTG G	19	56.7	53
M13rev-29	CAG GAA ACA GCT ATG ACC	18	53.7	50
M13rev-49	GAG CGG ATA ACA ATT TCA CAC AGG	24	61.0	46
M13uni-21	TGT AAA ACG ACG GCC AGT	18	53.7	50
M13uni-43	AGG GTT TTC CCA GTC ACG ACG TT	23	62.4	52
pBABE3	ACC CTA ACT GAC ACA CAT TCC	21	57.9	48
pBABE5	CTT TAT CCA GCC CTC AC	17	52.8	53
pBAD-FP	ATG CCA TAG CAT TTT TAT CC	20	51.1	35
pBakPAC-FP	TAA AAT GAT AAC CAT CTC GC	20	51.1	35
pBR1	CGA AAA GTG CCA CCT GAC	18	56	55.6
pBR3	TCC CCA TCG GTG ATG TC	17	55.2	58.8
pcDNA3.1-RP_1	CAA ACA ACA GAT GGC TGG C	19	56.7	52.6
pcDNA3_for	GGC TAA CTA GAG AAC CCA CTG	21	59.8	52
pcDNA3_rev	GGC AAC TAG AAG GCA CAG TC	20	59.3	55
pCEP-Forward	AGA GCT CGT TTA GTG AAC CG	20	57.3	50
pCEP-Reverse	GTG GTT TGT CCA AAC TCA TC	20	55.2	45
PCR2	TTA GCT CAC TCA TTA GG	17	47.9	41.2
pCR3.1-BGHrev	TAG AAG GCA CAG TCG AGG	18	56.0	56
pDONOR-FP	TAA CGC TAG CAT GGA TCT C	19	54.5	47.4
pDONOR-RP	GCA ATG TAA CAT CAG AGA T	19	50.2	36.8
pEGFP_for	TTT AGT GAA CCG TCA GAT C	19	52.4	42.1
pEGFP_rev	TTT AAA GCA AGT AAA ACC TC	20	49.1	30
pENTattL1for	TCG CGT TAA CGC TAG CAT GGA TCT C	25	64.6	52
pENTattL2rev	ACA TCA GAG ATT TTG AGA CAC GGG C	25	63.0	48
pET-24a	GGG TTA TGC TAG TTA TTG CTC AG	23	58.9	43.5
petup	ATG CGT CCG GCG TAG A	16	54.3	62
pEX-For	GGA GCA GAC AAG CCC GTC AGG	21	65.7	67
pEX-Rev	CAG GCT TTA CAC TTT ATG CTT CCG GC	26	64.8	50
pFASTBAC-F	TCC GGA TTA TTC ATA CCG TCC C	22	60.3	50
pFASTBAC-R	CCT CTA CAA ATG TGG TAT GGC TG	23	60.6	48
pGexfor	ATA GCA TGG CCT TTG CAG G	19	56.7	53
pGEX-3	GGA GCT GCA TGT GTC AGA GG	19	58.8	57.9
pGEX-5	CTG GCA AGC CAC GTT TGG	18	58.2	61.1
pGEX5-FP	AAC GTA TTG AAG CTA TCC C	19	52.4	42.1
pGLrev	CTT TAT GTT TTT GGC GTC TTC C	22	56.5	41
pGL3for	CTA GCA AAA TAG GCT GTC CC	20	57.3	50
pIRES-RP	TAT AGA CAA ACG CAC ACC G	19	54.5	47.4
pJET1.2for	CGA CTC ACT ATA GGG AGA GCG GC	23	66.0	61
pJET1.2rev	AAG AAC ATC GAT TTT CCA TGG CAG	25	66.0	52
pJET1_fwd	GCC TGA ACA CCA TAT CCA TCC	21	59.8	52
pJet1-FP	ACT ACT CGA TGA GTT TTC GG	20	55.3	45
pJet1-RP	TGA GGT GGT TAG CAT AGT TC	20	55.3	45
pLKO1	GAC TAT CAT ATG CTT ACC GT	20	53.2	40
pMalE	TCA GAC TGT CGA TGA AGC	18	53.7	50
Polyhed_fwd	AAA ATG ATA ACC ATC TCG	18	46.9	33
pQEfor	GTA TCA CGA GGC CCT TTC GTC T	22	62.1	55
pQErev	CAT TAC TGG ATC TAT CAA CAG GAG	24	59.3	42
pQE-FP	CGG ATA ACA ATT TCA CAC AG	20	53.2	40
pQE-RP	GTT CTG AGG TCA TTA CTG G	19	54.5	47.4
pRSET-RP	ATG CTA GTT ATT GCT CAG C	19	52.4	42.1
pTeSp-1	CCT CCA TAG AAG ACA CC	17	52.8	52.9
pTrcHis-RP	CTG ATT TAA TCT GTA TCA GG	20	51.1	35
SP6	CA TTT AGG TGA CAC TAT AG	19	50.2	37
T3	AAT TAA CCC TCA CTA AAG GG	20	53.2	40
T7	TAA TAC GAC TCA CTA TAG GG	20	53.2	40
T7term	CTA GTT ATT GCT CAG CGG T	19	54.5	47
T7-pET-mod	CCC GCG AAA TTA ATA CGA CTC AC	23	60.6	48
Topo-1	TCG GAT CCA CTA GTA ACG	18	53.7	50
U6_fwd	GAG GGC CTA TTT CCC ATG ATT CC	23	62.4	52.2
v5epitoperev	CGT AGA ATC GAG ACC GAG GAG AGG	24	66.1	58