Formula for precipitation. Not for enzymology or chromatography.
Use either Anhydrous or Trihydrate.
| Reagent | Amount | Fill with water |
|---|---|---|
| Sodium acetate Anhydrous (82.03 g/mol) |
410 mg |
49 ml |
| Sodium acetate Trihydrate (136.08 g/mol) |
680 mg |
49 ml |
Adjust pH with Acetic acid (glacial, molecular weight: 60.05 g/mol) by adding 290 ul observe the pH and if 5.2 to 5.5. pH fill up to 50 ml.