Strip buffer with formamide and 0.01% Triton X100.
Item | Item article number | Distributor | Vendor |
---|---|---|---|
Hi-Di™ Formamide |
4311320 |
ThermoFisher | Applied Biosystems |
10% Triton X100 | |||
UltraPure™ DNase/RNase-Free Distilled Water | 10977035 | ThermoFisher | Invitrogen |
Aliquot the formamide into Eppendorf tubes with 360 µl in each tube.
When you want to strip thaw the formamide and add 0.4 µl of 10% Triton X100
Add 39.6 µl of nuclease-free water and cortex.
Dont save aliquots. Throw away after use.